Publication date: November 2018Source:Renewable Energy, Volume 127 Author(s): Theresa Veeranan, Ramachandran Kasirajaan, Baskar Gurunathan, Renganathan Sahadevan In this present investigation, lipid extraction was performed in marine macro algae Ulva fasciata using soxhlet and super critical fluid extraction apparatus. The ultrasonic pretreatment was done for the algal biomass. Extraction parameters for High extraction yield of algal oil were obtained at 5% moisture content, 0.25 mm particle size, 90 °C, 180 min time, 5:1 solvent-to-solid ratio and solvent flow rate 1 l/hr. After the optimization process 9.85% of oil extraction was obtained from Ulva fasicata algal biomass. The aromatic groups, indicating the presence of 12-Hydroxy-9-octadecenoic acid was confirmed by FT-IR analysis. Thus the algal oil obtained from Ulva fasicata biomass can be used for the biodiesel production. The saturated fatty acid (SFAs) was found to be 75.43% in the produced algal oil. The properties like Acid value, Iodine number, specific gravity, Saponification value, calorific value, Flash point, Fire point and kinematic viscosity was also characterized.