Evaluation of organosolv pretreatment on the enzymatic digestibility of coconut coir fibers and bioethanol production via simultaneous saccharification and fermentation


Acidified organosolv solvent pretreatment was performed on coconut coir fibers.

Non-acid-catalyzed AG treatment resulted in low lignin removal and glucan digestibility.

AAG treatment with S/L ratio of 1:20 and 1:30 improved glucan digestibility to 81.8%.

8.97 g/L and 8.81 g/L of ethanol obtained from SSF under anaerobic condition.


Coconut coir fibers, which analyzed 34.8% glucan, 18.3% xylan, 35.8% lignin and 8.7% extractives, were pretreated with acidified aqueous glycerol (10% water) at a solid/liquid ratio loading of 1:20 and 1:30, a temperature of 130 °C and 60,30, and 15 min contact time. The maximum yield of glucan digestibility was 79.7% and 81.8% for the respective solid/liquid ratios for 30 min of pretreatment. The simultaneous saccharification and fermentation was conducted anaerobically at 37 °C with Saccharomyces cerevisiae, 5% w/v glucan, and 10 FPU/g glucan of cellulase. The highest ethanol concentrations obtained after 3 days of incubation from pretreated coconut coir fiber were 8.97 and 8.81 g/L, respectively, for the samples pretreated with acidified aqueous glycerol at 1:30 and 1:20 solid/liquid loading ratio for 30 min.

Graphical abstract


  • Coconut coir fibers;
  • Acidified glycerol pretreatment;
  • Lignin removal;
  • Glucan digestibility;
  • SSF

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