Optimization of various pretreatments condition of kenaf core (Hibiscus cannabinus) fibre for sugar production: Effect of chemical compositions of pretreated fibre on enzymatic hydrolysability


Pretreatments of kenaf core fibre for the enhancement of glucose yield production.

Optimization of pretreatments condition by RSM based on total glucose yield.

Effects of pretreatments on the chemical compositions of fibre were identified.

Removal of hemicellulose of core fibre improved hydrolysability most significantly.

Water pretreatment enhanced the enzymatic hydrolysability of fibre the most.


In this work, the effects of various pretreatments’ parameters on kenaf core fibre were analyzed statistically and optimized using Response Surface Methodology based on the total glucose yield. The chemical compositions of the pretreated fibres were examined to discuss the effect of pretreatment on the fibre hydrolysability comprehensively. The results showed that estimation model for each pretreatment of kenaf core fibre were polynomial equations. The optimum conditions for water, acid and alkali pretreatments were 170 °C for 45 min, 120 °C for 90 min in 2.0% H2SO4 solution and 140 °C for 60 min in 3.0% NaOH solution, respectively. Among the three pretreatments, water pretreatment achieved the highest total glucose yield (25.5%), followed by acid (20.0%) and alkali (18.2%) pretreatments. Based on chemical compositions analysis, both water and acid pretreatments were capable of eliminating almost 100% of hemicellulose with negligible removal of lignin while the alkali pretreatment removed both the lignin and hemicellulose more than 60%. This result revealed that the removal of hemicellulose showed greater influential in enhancing the enzymatic accessibility and hence, hydrolysability of kenaf core fibre.


  • Enzymatic hydrolysis;
  • Glucose;
  • Kenaf core fibre;
  • Optimization;
  • Pretreatment;
  • Response surface methodology

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